Dr. Jonathan Dattelbaum
Associate Professor of Chemistry
Co-Coordinator, Biochemistry & Molecular Biology Program
My research focuses on the design of fluorescent protein biosensors. Nature has provided a vast array of proteins which bind efficiently to molecules which my research group is interested in detecting. One of the goals of my group is to design a biosensor for glucose. The constructed optical biosensor may be used to measure glucose concentrations in complex media such as blood serum. A second main project in my lab, and in collaboration with Sabatino D'Auria (Institute for Protein Biochemistry, Naples, Italy), involves the characterization of an arginine binding protein from Thermotoga maritima. Finally, my lab collaborates with Dr. Malcolm Hill (UR Biology) on a chemical ecology project. Our goals are to isolate and characterize secondary metabolites from the symbiotic microbes associated with the Chesapeake Bay sponges, Clathria prolifera and Halichondria bowerbanki. We are interested in linking the biosynthetic genes and pathways with the corresponding microbial pigments in these organisms.
*Doan TT, *Vargo ML, *Gerig JK, *Gulka CP, Trawick ML, Dattelbaum JD, and Leopold ML. (2010) Electrochemical Investigation of Azurin Thermodynamic and Adsorption Properties at Monolayer-Protected Cluster Film Assemblies-Evidence for a More Homogeneous Adsorption Interface. Journal of Colloid & Interface Science, 352, 50-58.
Scirè, A., Marabotti, A., Staiano, M., Iozzino, L, *Luchansky, M.S., *Der, B.S., Dattelbaum, J.D., Tanfani, F., and D’Auria, S. (2010) Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritima. 2. Molecular organization and structural stability, Molecular Biosystems, 6, 687-698.
Dattelbaum, J.D., *Sieg, D., *Manieri, C.M., *Thomson, G., and Hill, M. (2010) Plasticity of acquired secondary metabolites in Clathria prolifera: Photoprotective role of carotenoids in a temperate intertidal sponge. The Open Marine Biology Journal, 87-95, DOI: 10.2174/1874450801004010087.
*Luchansky, M.S., *Der, B.S., D’Auria, S., Pocsfalvi, G., Iozzino, L., Marasco, D., and Dattelbaum, J.D. (2010) Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritima, Molecular BioSystems, 6,132-141, DOI: 10.1039/B908412F.
*Vargo, M.L., *Gulka, C.P., *Gerig, J.K., *Manieri, C.M., Dattelbaum, J.D., Marks, C., *Lawrence, N.T., Trawick, M.L., and Leopold, M.C. (2010) Distance Dependence of Electron Transfer Kinetics for Azurin Protein Adsorbed to Monolayer Protected Nanoparticle Film Assemblies. Langmuir, 26, 560-569, DOI: 10.1021/la9020367.
Dattelbaum, A.M., Baker, G.A., *Fox, J.M., Iyer, S., and Dattelbaum, J.D. (2009) Pegylation of a maltose biosensor promotes enhanced signal response when immobilized in a polymeric matrix. Bioconjugate Chemistry, 30, 2381-2384, DOI: 10.1021/bc900341s.
*Der, B.S. and Dattelbaum, J.D. (2008) Construction of a Reagentless Glucose Biosensor using Molecular Exciton Luminescence, Analytical Biochemistry, 375, 132-140.
*Vercillo, N.C., *Herald, K.J., *Fox, J.F., *Der, B.S., and Dattelbaum, J.D. (2007) Analysis of Ligand Binding to a Ribose Biosensor using Site-directed Mutagenesis and Fluorescence Spectroscopy. Protein Science,16, 362-368.
Dattelbaum, J.D., Looger, L.L, Benson, D.E., Sali, K.M., Thompson, R.B., and Hellinga, H.W. (2005) Analysis of Allosteric Signal Transduction Mechanisms in an Engineered Fluorescent Maltose Biosensor, Protein Science, 14, 284-291.